Dendritic cells (DCs) actively sample and present antigen to cells of the adaptive immune system and are thus vital for successful immune control and memory formation. Immune cell metabolism and function are tightly interlinked, and a better understanding of this interaction offers potential to develop immunomodulatory strategies. However, current approaches for assessing the immune cell metabolome are often limited by endpoint measurements, may involve laborious sample preparation, and may lack unbiased, temporal resolution of the metabolome. In this study, we present a novel setup coupled to a secondary electrospray ionization-high resolution mass spectrometric (SESIHRMS) platform allowing headspace analysis of immature and activated DCs in real-time with minimal sample preparation and intervention, with high technical reproducibility and potential for automation.

Improving the diagnosis and management of pediatric asthma is necessary. Breath analysis can help by assessing altered metabolism and disease-associated processes in a non-invasive manner. This study aimed to identify exhaled metabolic signatures using SUPER SESI - HRMS that can distinguish children with allergic asthma from healthy controls.

This application note highlights the potential of breath analysis in providing valuable insights into the exposure of lungs and the absorption of molecules directly into the bloodstream. The SUPER SESI - QExactive HF -EXHALION system was utilized for breath sample analysis, which recorded a volunteer's breath for 20 minutes after smoking a cigarette. The study captured insights into the kinetics of tobacco metabolites. This approach underscores the capability of breath analysis to provide real-time and valuable data on substance metabolism and exposure. It showcases a significant step forward in non-invasive diagnostic methodologies.

This study explores breath analysis as a tool for therapeutic drug monitoring (TDM) in clinical settings, focusing on valproic acid (VPA) in epilepsy treatment. It contrasts real-time and off-line mass spectrometry methods for analyzing exhaled compounds associated with VPA. Real-time analysis involves direct exhalation into a mass spectrometer, while off-line analysis uses a gas collection device.

Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state-of-the-art diagnostics.

We are thrilled to announce a fantastic job opening at Diagnose Early (www.diagnoseearly.com), a pioneer in advanced technological research. Thanks to recent strategic investments, including the acquisition of several state-of-the-art SUPER SESI - Orbitrap breath analysis machines, DE is at the forefront of biomarker identification research.

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In this study, SUPER SESI-Orbitrap MS was used to analyze the time-resolved gas-phase volatile organic compounds (VOCs) of garlic, ramsons, and onion.

The aim of this study was to address the difficulties of characterizing heat-labile sulfur molecules from various Allium spp. In this, we will delve into the analytical method SUPER SESI-HRMS used in this study and its applications in the analysis of volatile compounds in garlic, onion, and ramsons.

SUPER SESI-HRMS was used to non-invasively and in real-time monitor the pharmacokinetics of ketamine, propofol, and valproic acid, along with their metabolites in mice. This approach allowed for obtaining real-time pharmacokinetic curves without the need to sacrifice the animals, representing a significant advance in terms of animal welfare and time and cost savings. 

A few days ago, we attended a demonstration organized by FossilionTech in the laboratories of Thermo Fisher Scientific in Paris. The performance of the SUPER SESI X-Orbitrap Exploris 120 in the in vitro and in vivo analysis of volatile organic compounds (VOCs) present in food was showcased. The primary goal of this event was to demonstrate the ability of SUPER SESI X-ORBITRAP MS to detect and monitor Volatile Organic Compounds (VOCs) in food, emphasizing the importance of incorporating this technology into food production processes for a deeper understanding of the aromas and flavors of food. Additionally, it highlights the significant improvements in food safety that can be achieved through the adoption of this technology.

Hendrik Mengers, Martin Zimmermann & Lars M. Blank from Aachen University monitored the volatilome of yeast during growth in a shake flasks bioreactor. Among many more metabolites, they caught the evolution of acetaldehyde and ethanol within seconds.

With a 0.1 nA precision current monitoring and 100% automatic control, the SSX makes high-performance nano-electrospray truly easy to operate.

We had to adjust the mechanical latches and the sealing because this was the first time we coupled an SSX to a Q Fortis MS. Thanks to Kseniya Dryahina, Patrik Španěl, Radim from Pragolab s.r.o., and the team at the J. Heyrovský Institute of Physical Chemistry CAS, this was completed in one day, and we were happily acquiring breath and headspace data on the second day :)

Thanks to SuperSESI we can finally see, accurately and in real time, what happens in our lungs after smoking.

This work from the Ohio State University shows the potential of the platform SUPER SESI (by Fossiliontech) - Orbitrap (by Thermo Fisher Scientific) on differentiating cancer types and monitoring their treatment.

Electrospray provides the best performance, but obtaining a stable signal can be tricky.
The new SUPER SESI-X, designed for the Thermo Orbitrap Exploris series, is fully automatic and is ready to incorporate intelligent control. This source will auto-pilot the electrospray to bring high performance and reliability.

We have developed this first pilot unit within the PERFECT LIFE project (funded by the European Union). It comprises a gas conditioning unit, a desorber, the Super SESI, the MS, and of course, the cartridges!

You can now use the SimpleLINK UNO-16 even simpler!
For those who use 1/16" fittings, SimpleLINK UNO-16 can be your best friend to connect the column and the emitter.

We are developing several peripheral systems to expand how you can use Super SESI to your advantage.

Dr. Mike Wilde, of the East Midlands Breathomics Pathology Node (EMBER) Council, has been awarded this SUPER SESI of the "Together we fight COVID" program for his brilliant project proposal "Breath analysis by SESI-HRMS within a multi-omic platform for patient stratification in COVID-19 pneumonia".

Guillermo Vidal-de-Miguel, our CEO, explains it in detail! We hope you enjoy it ;)