Biomarker

Molecular breath analysis supports altered amino acid metabolism in idiopathic pulmonary fibrosis

Molecular breath analysis supports altered amino acid metabolism in idiopathic pulmonary fibrosis

Martin Thomas Gaugg, Anna Engler, Lukas Bregy, Yvonne Nussbaumer-Ochsner, Lara Eiffert, Tobias Bruderer, Renato Zenobi, Pablo ML Sinues and Malcolm Kohler.

Background
Collagen-related amino acids are significantly increased in exhaled breath of idiopathic pulmonary fibrosis (IPF) patients compared with healthy controls. The detection of these amino acids using real-time breath analysis results in a good discrimination between the groups, indicating the possibility for a rapid, non-invasive screening for IPF.

Real-time breath analysis reveals specific metabolic signatures of COPD exacerbations

Real-time breath analysis reveals specific metabolic signatures of COPD exacerbations

Martin Thomas Gaugg, Yvonne Nussbaumer-Ochsner, Lukas Bregy, Anna Engler, Nina Stebler, Thomas Gaisl, Tobias Bruderer, Nora Nowak, Pablo ML Sinues, Renato Zenobi and Malcolm Kohler.

Background
Exacerbations of chronic obstructive pulmonary disease (COPD) are defined by acute worsening of respiratory symptoms leading to a change in therapy.

Real-time exhaled breath analysis in patients with cystic fibrosis and controls

Real-time exhaled breath analysis in patients with cystic fibrosis and controls

T. Gaisl, L. Bregy, N. Stebler, M. T. Gaugg, T. Bruderer, D. García-Gómez, A, Moeller,  F. Singer,  E. I.  Schwarz, C. Benden, P. M-L Sinues, R. Zenobi, M. Kohler

We aimed at defining profiles of volatile organic compounds in exhaled breath from patients with cystic fibrosis (CF) using a novel real-time mass spectrometry technique. In this prospective matched case-control study, 30 patients with CF, and 30 healthy control 4 subjects were matched one-to-one according to age, gender, and smoking state.

Real-time mass spectrometric identification of metabolites characteristic of chronic obstructive pulmonary disease in exhaled breath

Real-time mass spectrometric identification of metabolites characteristic of chronic obstructive pulmonary disease in exhaled breath

L. Bregya, Y Nussbaumer-Ochsnerb, P. M-L Sinues, D. García-Gómez, Y. Suter, T. Gaisl, N. Stebler, M. T. Gaugg, M. Kohler, R. Zenobi

New mass spectrometry (MS) techniques analysing exhaled breath have the potential to better define airway diseases. Here, we present our work to profile the volatile organic compounds (VOCs) in exhaled breath from patients with chronic obstructive pulmonary disease (COPD), using real-time MS, and relate this disease-specific breath profile to functional disease markers …

METHOD OF DETECTING COPD BY MASS SPECTROMETRY

METHOD OF DETECTING COPD BY MASS SPECTROMETRY

L. Bregya, D. García-Gómez, M. Kohler, Y Nussbaumer-Ochsnerb, P. M-L Sinues, Y. Suter, R. Zenobi, J. Schmitz

The present invention provides a method for determining whether a patient suffers from COPD, said method comprising the step of: a. providing at a sample comprising, or consisting of, exhaled breath of said patient to an ionization chamber of a mass spectrometer; b. simultaneously determining the presence, absence or relative concentration of at least two metabolites in said sample using …

Mass-spectrometric detection of omega-oxidation products of aliphatic fatty acids in exhaled breath

Mass-spectrometric detection of omega-oxidation products of aliphatic fatty acids in exhaled breath

M. T. Gaugg, T. Bruderer, N. Nowak, L. Eiffert, P. M-L Sinues, M. Kohler, R. Zenobi

Omega-oxidation is a fatty acid degradation pathway that can occur alternatively to the dominant b-oxidation. The dysregulation of fatty acid oxidation has been related with a variety of diseases, termed fatty acid oxidation disorders. This work shows evidence for real-time detection in exhaled breath of the complete series of saturated linear w-hydroxyalkanoic acids, w-oxoalkanoic acids and alkanedioic acids with carbon chain lengths of 5-15.

SESI-MS feasibility study with infants and young children for early detection of cystic fibrosis inflammation and infection

SESI-MS feasibility study with infants and young children for early detection of cystic fibrosis inflammation and infection

T. Bruderer, A. Baghdasaryan, J. Wyler, M. Kohler, R. Zenobi, A. Möller

Early and often subclinical pulmonary infection and pronounced neutrophilic inflammation are major contributors to CF-related morbidity. There is a causal relationship between high airway neutrophil elastase activity and the development of bronchiectasis. Early detection of disease and disease-associated complications is crucial for implementing timely therapeutic measures …

Targeted on-line breath analysis discriminates COPD patients vs. healthy controls and subjects suffering from asthma

Targeted on-line breath analysis discriminates COPD patients vs. healthy controls and subjects suffering from asthma

Y. Nussbaumer-Ochsner, M.T. Gaugg, L. Bregy, A. Engler, S. A. Sophie, T. Gaisl, P. M-L Sinues, M. Kohler, R. Zenobi

Recently we found markers in exhaled breath discriminating patients with chronic obstructive pulmonary disease (COPD) from healthy controls using real-time mass spectrometry. The aim of this study was to validate the previously found disease specific metabolic profile of COPD in an independent cohort of patients suffering from chronic obstructive lung disease …

Exhaled breath analysis by real-time Mass spectrometry in patients with pulmonary fibrosis

Exhaled breath analysis by real-time Mass spectrometry in patients with pulmonary fibrosis

P. M-L Sinues, Y. Nussbaumer Ochsner, M.T. Gaugg, L. Bregy, A. Engler, R. Zenobi, M. Kohler.

Idiopathic pulmonary fibrosis (IPF) is recognized as a distinct clinical disorder, however, the diagnosis method remains elusive. Metabolic profiling of biopsied tissue specimens has shown promise to gain insights into IPF pathogenesis. In view of this, the authors hypothesized that the analysis of exhaled metabolites may also provide further insights.

二次纳流电喷雾电离耦合 超高分辨质谱检测人体呼出气中 相对高分子量化合物

黄 磊, 李 雪, 徐 萌, 黄正旭, 周 振

二次电喷雾电离源耦合超高分辨质谱(SESI⁃UHRMS)有望检出人体呼出气中分子量大于300 的相对 高分子量化合物, 这些化合物的发现将有助于更准确地理解呼出气中挥发性有机化合物(VOCs)的来源、产 生机制以及SESI 源电离机理, 更好地实现SESI⁃UHRMS 的转化应用. 本研究自组装nanoSESI 源(尚无商业产 品)耦合四极杆⁃静电场轨道阱质谱(最高质量分辨率1.2×10<sup>5</sup> ), 考察了该装置对健康人体呼出气中分子量 为300~500 化合物的检出情况. 结果表明, 所搭建的nanoSESI⁃UHRMS 装置检测人体呼出气的重现性好、灵 敏度高, 可检出数十种分子量为300~500 的化合物.

On-line breath analysis with secondary electrospray ionization discriminates between COPD patients with and without frequent exacerbations

On-line breath analysis with secondary electrospray ionization discriminates between COPD patients with and without frequent exacerbations

M. T. Gaugg, Y. Nussbaumer-Ochsner, L. Bregy, A. Engler, N. Stebler, T. BrudererP. M-L Sinues, R. Zenobi, M. Kohler

The authors successfully identified metabolic patterns in exhaled breath, which discriminate COPD patients with and without frequent exacerbations. They propose that their findings correlate with the increased oxidative stress caused by elevated nitric oxide production in response to the pulmonary inflammation.

SESI-HRMS reveals tryptophan pathway metabolites in exhaled human breath

SESI-HRMS reveals tryptophan pathway metabolites in exhaled human breath

D. García-Gómez, T. Gaisl, L. Bregy, P. M-L Sinues, M. Kohler and R. Zenobi

Disorders in tryptophan metabolism result in diseases such as vitamin B6 responsive xanthurenic aciduria, hydroxy-kynureninuria, tryptophanuria and Hartnup disease. The authors hypotesized that breath analysis may reveal compounds that are metabolically linked to tryptophan. The mass range of detected metabolites reached up to 265 u, which is beyond the mass range limit (150–200 u) of competing breath research techniques such as PTR or SIFT-MS.

Expanding metabolite coverage of real-time breath analysis by coupling a universal SESI source and a HRMS. A pilot study on tobacco smokers

Expanding metabolite coverage of real-time breath analysis by coupling a universal SESI source and a HRMS. A pilot study on tobacco smokers

M. T. Gaugg, D. Garcia Gomez, C. Barrios-Collado, G. Vidal-de-Miguel, M. Kohler, R. Zenobi and P. M-L Sinues

Coupling a SESI source and a HRMS (Orbitrap), the authors were able to identify exogenous compounds associated to smoking, as well as endogenous metabolites suggesting increased oxidative stress in smokers. According to the authors, most of these compounds correlated significantly with smoking frequency and allowed accurate discrimination of smokers and non-smokers.