While yeast is one of the most studied organisms, its intricate biology remains to be fully mapped and understood. This is especially the case when it comes to capture rapid, in vivo fluctuations of metabolite levels. According to the authors, the results suggest that a large number of metabolites produced by yeast from glucose neither are reported in the literature nor are their biochemical origins deciphered.
The authors explore whether real-time breath research & analysis by SESI-HRMS is suitable to monitor changes at the metabolic level due to inhaling bronchodilator medication. The experimental results strengthen the notion that certain biochemical processes can be monitored.
Omega-oxidation is a fatty acid degradation pathway that can occur alternatively to the dominant b-oxidation. The dysregulation of fatty acid oxidation has been related with a variety of diseases, termed fatty acid oxidation disorders. This work shows evidence for real-time detection in exhaled breath of the complete series of saturated linear w-hydroxyalkanoic acids, w-oxoalkanoic acids and alkanedioic acids with carbon chain lengths of 5-15.
I. A. Ratiu, T. Ligor, Victor B.-Bintintan, B. Buszewski
Bacteria are the main cause of many human diseases. Typical bacterial identification methods, for example culture-based, serological and genetic methods, are time-consuming, delaying the potential for an early and accurate diagnosis and the appropriate subsequent treatment.
A. T. Rioseras, M. T. Gaugg and P. M-L Sinues
Our main goal was to gain further insights into the mechanism by which gas-phase analytes are ionized by interaction with plumes of electrospray solvents. We exposed target vapors to electrosprays of either water or deuterated water and mass analyzed them …
Early and often subclinical pulmonary infection and pronounced neutrophilic inflammation are major contributors to CF-related morbidity. There is a causal relationship between high airway neutrophil elastase activity and the development of bronchiectasis. Early detection of disease and disease-associated complications is crucial for implementing timely therapeutic measures …
Y. Nussbaumer-Ochsner, M.T. Gaugg, L. Bregy, A. Engler, S. A. Sophie, T. Gaisl, P. M-L Sinues, M. Kohler, R. Zenobi
Recently we found markers in exhaled breath discriminating patients with chronic obstructive pulmonary disease (COPD) from healthy controls using real-time mass spectrometry. The aim of this study was to validate the previously found disease specific metabolic profile of COPD in an independent cohort of patients suffering from chronic obstructive lung disease …
P. M-L Sinues, Y. Nussbaumer Ochsner, M.T. Gaugg, L. Bregy, A. Engler, R. Zenobi, M. Kohler.
Idiopathic pulmonary fibrosis (IPF) is recognized as a distinct clinical disorder, however, the diagnosis method remains elusive. Metabolic profiling of biopsied tissue specimens has shown promise to gain insights into IPF pathogenesis. In view of this, the authors hypothesized that the analysis of exhaled metabolites may also provide further insights.
黄 磊， 李 雪， 徐 萌， 黄正旭， 周 振
二次电喷雾电离源耦合超高分辨质谱（ＳＥＳＩ⁃ＵＨＲＭＳ）有望检出人体呼出气中分子量大于３００ 的相对 高分子量化合物， 这些化合物的发现将有助于更准确地理解呼出气中挥发性有机化合物（ＶＯＣｓ）的来源、产 生机制以及ＳＥＳＩ 源电离机理， 更好地实现ＳＥＳＩ⁃ＵＨＲＭＳ 的转化应用． 本研究自组装ｎａｎｏＳＥＳＩ 源（尚无商业产 品）耦合四极杆⁃静电场轨道阱质谱（最高质量分辨率１.２×1０<sup>５</sup> ）， 考察了该装置对健康人体呼出气中分子量 为３００～５００ 化合物的检出情况． 结果表明， 所搭建的ｎａｎｏＳＥＳＩ⁃ＵＨＲＭＳ 装置检测人体呼出气的重现性好、灵 敏度高， 可检出数十种分子量为３００～５００ 的化合物．
The authors successfully identified metabolic patterns in exhaled breath, which discriminate COPD patients with and without frequent exacerbations. They propose that their findings correlate with the increased oxidative stress caused by elevated nitric oxide production in response to the pulmonary inflammation.
HUANG Lei, LI Xue, XU Meng, HUANG Zhengxu, ZHOU Zhen
Human breath contains thousands of volatile organic compounds (VOCs), which would be potentially helpful for studying disease diagnosis and environmental exposure. However, owing to limitations in current analytical methods, only the compounds with molecular weights <300 have been widely reported in exhaled human breath.
R. R. Farrell, J. Fahrentrapp, D. Garcia Gomez, P. M-L Sinues, and R. Zenobi
Even though sugar and acidity measurements are the most common indices of grape maturity, it is well recognized that they provide only basic information related to wine quality. In this preliminary study te authors use SESI-MS to analyze VOCs directly from intact grapes without sample concentration.
P. M-L Sinues, M. Kohler, S. A. Brown, R. Zenobi and R. Dallmann
The time-of-day of drug application is an important factor in maximizing efficacy and minimizing toxicity. Real-time in vivo mass spectrometric breath analysis and research of mice was deployed to investigate time-of-day variation in ketamine metabolism. Different production rates of ketamine metabolites were found in opposite circadian phases.