Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry

Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry

L. Zhu, G. Gamez, H. W. Chen, H. X. Huang, K. Chingin and Renato Zenobi

Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry.png

Abstract

Extractive electrospray ionization mass spectrometry (EESI-MS) for real-time monitoring of organic chemical reactions was demonstrated for a well-established pharmaceutical process reaction and a widely used acetylation reaction in the presence of a nucleophilic catalyst, 4-dimethylaminopyridine (4-DMAP).

EESI-MS provides real-time information that allows us to determine the optimum time for terminating the reaction based on the relative intensities of the precursors and products. In addition, tandem mass spectrometric (MS/MS) analysis via EESI-MS permits on-line validation of proposed reaction intermediates. The simplicity and rapid response of EESI-MS make it a valuable technique for on-line characterization and full control of chemical and pharmaceutical reactions, resulting in maximized product yield and minimized environmental costs.

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