Applications of High-Resolution Mass Spectrometry in Metabolomics
M. Fido
Abstract
Expanding high-resolution mass spectrometry applications in metabolomics
This doctoral thesis explores the use of high-resolution mass spectrometry to address diverse challenges in metabolomics, ranging from volatile compound analysis to targeted quantification and computational data processing. Across four studies, the work investigates polymer sampling bags for offline breath analysis, applies volatilomics to study the metabolic effects of gut microbiota and pathogen infection in honey bees, develops an HRMS-based method for the detection and quantification of nitrogen-containing metabolites, and introduces LCMSpector, an open-source tool for targeted mass spectrometry data analysis. Together, these contributions demonstrate the versatility of high-resolution mass spectrometry for studying complex biological matrices, improving analytical workflows, and supporting more reliable metabolomic interpretation.