Neutral desorption sampling coupled to extractive electrospray ionization mass spectrometry for rapid differentiation of biosamples by metabolomic fingerprinting
H. Chen, A. Wortmann, Renato Zenobi
It is of increasing interest and practical importance to develop convenient methods based on mass spectrometry for high-throughput analyses of biological samples. This is usually difficult because of the complex matrix and ion suppression effects. Generation of ions at ambient conditions is a promising solution to these problems …
Differentiation of Maturity and Quality of Fruit Using Noninvasive Extractive Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry
H. Chen, Y. Sun, A. Wortmann, H. Gu, R. Zenobi
Maturity is an essential factor that determines storage life and final quality of most fruits and vegetables. Maturity monitoring is thus of paramount importance for postharvest handling and fruit quality regulation. Ideal analytical procedures for maturity investigation require high sensitivity, specificity, and high throughput and should be noninvasive.
Electrospray ionization of volatiles in breath
P. M-L Sinues, J. F. de la Mora
Recent work by Zenobi and colleagues reports that human breath charged by contact with an electrospray (ES) cloud yields many mass peaks of species such as urea, glucose, and other ions, some with molecular weights above 1000Da.
Manipulation of charge states of biopolymer ions by atmospheric pressure ion/molecule reactions implemented in an extractive electrospray ionization source
H. Chen, D. Touboul,M. C. Jecklin, J. Zheng, M. Luoa, Renato Zenobid
A home-made extractive electrospray ionization source is coupled to a linear quadrupole ion trap mass spectrometer to investigate ion/molecule reactions of biopolymers at ambient pressure. Multiply charged biopolymers such as peptides and proteins generated in an electrospray are easily reduced to a low charge state by …
Rapid In Vivo Fingerprinting of Nonvolatile Compounds in Breath by Extractive Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry
H. Chen, A. Wortmann, W. Zhang, and R. Zenobi
Quantitative analysis of trace constituents in exhaled gas can provide useful insights into biochemical processes in the body, thus revealing information about metabolic dynamics and providing, theoretically, a scientific base for biomarker research or clinical diagnoses. Breath, however, is rarely used practically for diagnostic purposes in clinical medicine …
Secondary Electrospray Ionization-Ion Mobility Spectrometry for Explosive Vapor Detection
M. Tam and H. H. Hill, Jr.
The unique capability of secondary electrospray ionization (SESI) as a nonradioactive ionization source to detect analytes in both liquid and gaseous samples was evaluated using aqueous solutions of three common military explosives: cyclo-1,3,5-trimethylene-2,4,6-trinitramine (RDX), nitroglycerin (NG) and pentaerythritol tetranitrate (PETN).
Secondary Ionization of Chemical Warfare Agent Simulants: Atmospheric Pressure Ion Mobility Time-of-Flight Mass Spectrometry
W. E. Steiner, B. H. Clowers, P. E. Haigh, and H. H. Hill
For the first time, the use of a traditional ionization source for ion mobility spectrometry (radioactive nickel (63Ni) beta emission ionization) and three alternative ionization sources (electrospray ionization (ESI), secondary electrospray ionization (SESI), and electrical discharge (corona) ionization (CI)) were employed with …
Secondary Electrospray Ionization Ion Mobility Spectrometry/Mass Spectrometry of Illicit Drugs
C. Wu, W. F. Siems, and H. H. Hill, Jr.
A secondary electrospray ionization (SESI) method was developed as a nonradioactive ionization source for ion mobility spectrometry (IMS). This SESI method relied on the gas-phase interaction between charged particles created by electrospray ionization (ESI) and neutral gaseous sample molecules. Mass spectrometry (MS) was used as …