S.M. Ashbacher D.C. Muddiman

Heat-Stress VOC Profiling in Basil Using TP-SESI
This study showcases temperature-programmed secondary electrospray ionization (TP-SESI) as a powerful tool for probing plant responses to heat stress. By applying controlled heating to Ocimum basilicum leaves, TP-SESI enabled real-time, non-destructive monitoring of volatile organic compounds (VOCs). The results revealed clear, temperature-dependent changes in VOC composition and abundance, offering insights into thermal stress signaling pathways. TP-SESI emerges as a sensitive, orthogonal method for exploring plant resilience mechanisms and stress-induced metabolism.

Kim Arnold, Alejandro Gómez-Mejia, Miguel de Figueiredo, An N. T. Phan, Roy Eerlings, Hendrik G. Mengers & Lars M. Blank

Here, we demonstrated the secondary electrospray ionization coupled Orbitrap mass spectrometry (SESI-Orbitrap MS) methodology for quantifying tulipalin A release from plants upon injury.

C. Barrios-Collado, D. Garcia-Gomez, R. Zenobi, G. Vidal-de-Miguel, Alfredo J. Ibanez, and P. M-L Sinues

The authors document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day−night cycles. To further illustrate the capabilities of SESI-MS for volatile organic compounds (VOCs) analysis, they subjected the plant to mechanical damage and monitored its response.