2015

The Effect of CPAP Withdrawal on Exhaled Breath in Obstructive Sleep Apnea – A Randomized Controlled Trial

The Effect of CPAP Withdrawal on Exhaled Breath in Obstructive Sleep Apnea – A Randomized Controlled Trial

E. I Schwarz, P. M-L Sinues, L. Bregy, T. Gaisl, D. Garcia-Gomez, M. T. Gaugg, Y. Suter, N. Stebler, Y. Nussbaumer-Ochsner, K. E. Bloch, J. R. Stradling, R. Zenobi, M. Kohler

Patients with OSA effectively treated with CPAP were randomised to either withdraw treatment or continue CPAP for 2 weeks. Exhaled breath analysis and research by untargeted SESI-MS was performed at baseline and 2 weeks after randomisation. CPAP withdrawal led to a recurrence of OSA, which was accompanied by a significant change in 16 identified metabolites.

Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization – mass spectrometry

Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization – mass spectrometry

L. Bregy, A. R. Müggler, P. M-L Sinues, D. García-Gómez, Y. Suter, G. N. Belibasakis, M. Kohler, P. R. Schmidlin, Renato Zenobi

The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer …

Detection and Quantification of Benzothiazoles in Exhaled Breath and Exhaled Breath Condensate by Real-Time SESI-HRMS and UHPLC

Detection and Quantification of Benzothiazoles in Exhaled Breath and Exhaled Breath Condensate by Real-Time SESI-HRMS and UHPLC

D. García-Gómez, L. Bregy, Y. Nussbaumer-Ochsner, T. Gaisl, M. Kohler, and R. Zenobi

2-Subtituted benzothiazoles are widely used industrial chemicals whose occurrence in environmental samples has been shown to be ubiquitous. However, knowledge about human exposure to these compounds and their excretion route is still scarce. Here, we demonstrate for the first time the detection of benzothiazole derivatives in exhaled breath.

SESI-MS and a novel statistical bioinformatic approach identifies a cancer-related profile in exhaled breath of breast cancer patients: a pilot study

SESI-MS and a novel statistical bioinformatic approach identifies a cancer-related profile in exhaled breath of breast cancer patients: a pilot study

P. M-L Sinues, Elena Landoni, Rosalba Miceli, Vincenza F. Dibari, Matteo Dugo, Roberto Agresti, Elda Tagliabue, Simone Cristoni, Rosaria Orlandi.

SESI–MS produces high-dimensional data of quality comparable to gene expression analysis output. This prompted the authors to use methods originally developed for gene expression data in analyzing SESI–MS breathprints.

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

C. Barrios-Collado, G. Vidal-de-Miguel, P. M-L Sinues

The process by which ambient vapors are ionized upon interaction with electrosprays is not fully under-stood, compromising its optimization and widespread use. In this work we evaluated the different scales associated with the processes involved in secondary electrospray ionization (SESI) and developed a new numerical method …

Real-Time High-Resolution Tandem Mass Spectrometry Identifies Furan Derivatives in Exhaled Breath

Real-Time High-Resolution Tandem Mass Spectrometry Identifies Furan Derivatives in Exhaled Breath

D. García-Gómez, L. Bregy, C. Barrios-Collado, G. Vidal-de-Miguel and R. Zenobi

The identification of chemical compounds in exhaled human breath is promising in the search for new biomakers of diseases. However, the analytical techniques used nowadays are not capable of achieving a robust identification, especially in real-time analysis.

Drug Pharmacokinetics Determined by Real-Time Analysis of Mouse Breath

Drug Pharmacokinetics Determined by Real-Time Analysis of Mouse Breath

Xue Li, P. M-L Sinues, R. Dallmann, L. Bregy, M.Hollmén, S. Proulx, S. A. Brown, M. Detmar, M. Kohler, and R. Zenobi

Noninvasive, real-time pharmacokinetic (PK) monitoring of ketamine, propofol, and valproic acid, and their metabolites was achieved in mice, using secondary electrospray ionization and high-resolution mass spectrometry. The PK profile of a drug influences its efficacy and toxicity because it determines exposure time and levels …

Profiling Aged Artisanal Cheddar Cheese Using Secondary Electrospray Ionization-Mass Spectrometry (SESI-MS)w

Profiling Aged Artisanal Cheddar Cheese Using Secondary Electrospray Ionization-Mass Spectrometry (SESI-MS)w

H. D. Bean, T. R. Mellors, J. Zhu and J. E. Hill

A number of direct injection mass spectrometry methods that can sample foods nondestructively and without sample preparation are being developed with applications ranging from the rapid assessment of food safety to the verification of protected designations of origin. In this pilot study, SESI-MS in positive and negative-ion modes was used to …

Identification of 2‑Alkenals, 4‑Hydroxy-2-alkenals, and 4‑Hydroxy-2,6-alkadienals in Exhaled Breath Condensate by UHPLC-HRMS and in Breath by Real-Time HRMS

Identification of 2‑Alkenals, 4‑Hydroxy-2-alkenals, and 4‑Hydroxy-2,6-alkadienals in Exhaled Breath Condensate by UHPLC-HRMS and in Breath by Real-Time HRMS

D. García-Gómez, P. M.-L. Sinues, C. Barrios-Collado, G. Vidal-de Miguel, M. Gaugg, and R. Zenobi

In recent years, breath analysis in real time has become a noninvasive alternative for the diagnosis of diseases and for molecular fingerprinting of exhaled breath. However, the techniques used lack the capabilities for proper identification of the compounds found in the exhalome.