2014

Circadian Variation of the Human Metabolome Captured by Real-Time Breath Analysis

Circadian Variation of the Human Metabolome Captured by Real-Time Breath Analysis

P. M-L Sinues, L. Tarokh, Xue Li, M. Kohler, S. A. Brown, R. Zenobi, R. Dallmann

Circadian clocks play a significant role in the correct timing of physiological metabolism, and clock disruption might lead to pathological changes of metabolism. One interesting method to assess the current state of metabolism is metabolomics. Metabolomics tries to capture the entirety of small molecules, i.e. the building blocks of metabolism, in a given matrix …

Direct quantification of chemical warfare agents and related compounds at low ppt levels: comparing active capillary DBDI and SESI mass spectrometry

Direct quantification of chemical warfare agents and related compounds at low ppt levels: comparing active capillary DBDI and SESI mass spectrometry

J-C Wolf, M. Schaer, P. Siegenthaler, R. Zenobi

A novel active capillary dielectric barrier discharge plasma ionization (DBDI) technique for mass spectrometry is applied to the direct detection of thirteen chemical warfare related compounds, including sarin, and compared to secondary electrospray ionization (SESI) in terms of selectivity and sensitivity.

Identifying methicillin-resistant Staphylococcus aureus (MRSA) lung infections in mice via breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS)

Identifying methicillin-resistant Staphylococcus aureus (MRSA) lung infections in mice via breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS)

H. D. Bean, J. Zhu, J. C. Sengle and J. E. Hill

Invasive methicillin-resistant Staphylococcus aureus (MRSA) infections are a serious health threat, causing an estimated 11000 deaths per year in the United States. MRSA pneumonias account for 16% of invasive infections, and can be difficult to detect as the current state-ofthe-art diagnostics require that bacterial DNA is recovered from the infection site …

Fingerprinting Breast Cancer vs. Normal Mammary Cells by Mass Spectrometric Analysis of Volatiles

Fingerprinting Breast Cancer vs. Normal Mammary Cells by Mass Spectrometric Analysis of Volatiles

Jingjing He, P. M-L Sinues, M. Hollmén, Xue Li, M. Detmar & R. Zenobi

There is increasing interest in the development of noninvasive diagnostic methods for early cancer detection, to improve the survival rate and quality of life of cancer patients. Identification of volatile metabolic compounds may provide an approach for noninvasive early diagnosis of malignant diseases.

Rapid identification of bacteria in blood cultures by mass-spectrometric analysis of volatiles

Rapid identification of bacteria in blood cultures by mass-spectrometric analysis of volatiles

C. Ballabio, S. Cristoni, G. Puccio, M. Kohler, M. R. Sala, P. Brambilla, P. M-L Sinues

Blood cultures are routine tests to determine whether micro-organisms have entered the patient’s bloodstream. Automated systems, based on the detection of CO2 increase in the culture media, have considerably improved the screening efficiency for the detection of bacteria. However, further identification of bacteria still requires time consuming culturing procedures.

Breath Analysis in Real Time by Mass Spectrometry in Chronic Obstructive Pulmonary Disease

Breath Analysis in Real Time by Mass Spectrometry in Chronic Obstructive Pulmonary Disease

P. M-L Sinues, L. Meier, C. Berchtold, M.Ivanov, N. Sievi, G. Camen, M. Kohler, R. Zenobi

Background: It has been suggested that exhaled breath contains relevant information on health status. Objectives: We hypothesized that a novel mass spectrometry (MS) technique to analyze breath in real time could be useful to differentiate breathprints from chronic obstructive pulmonary disease (COPD) patients and controls (smokers and nonsmokers) …