SUPER SESI-QE
SSQE
REAL-TIME VOLATILOMICS
SS-QE is a high efficiency Secondary Electrospray Ionization (SESI) source optimized for the analysis of volatile and semi-volatile organic compounds in real-time. SS-QE is compatible with Thermo Scientific high resolution Mass Spectrometers: LTQ, Exactive and Q-Exactive Orbitrap series.
How it works:
Vapors are preheated and mixed with a cloud of protonated water clusters produced by the nano-electrospray.
Vapors are ionized at high pressure to maximize ionization efficiency and ions are transferred to the MS for the analysis.
HIGHLY RELEVANT METABOLITES in the gas phase.
Low volatility species tend to be more relevant from a biological standpoint. These larger molecules carry more information, but they are often very diluted because they have a low vapor pressure.
BIOLOGICAL RELEVANT MOLECULES:
SESI is inherently very efficient. By operating at atmospheric pressure, the ions and the vapors of interest are very concentrated. Also, coulombic repulsion and dilution of newly formed ions are much lower than at reduced pressures. For a given sample, a better ionization efficiency means more ions being transferred to the mass spectrometer.
Harnessing the power of the best mass spectrometers in the market: best ionization + best ion analysis = best results.
Improving background levels: low volatility species tend to condensate in the ionizer. Handling them properly is essential.
Cleaning: Ideally, the system should not get contaminated, but the truth is that contamination happens. SUPER SESI automates some cleaning procedures and facilitates deep cleaning.
Maintaining a stable electrospray without fragmentation: this greatly simplifies the structure of the resulting data.
Customer Success
Dr. Prof. Patrik Španěl - J. Heyrovsky Institute of Physical Chemistry of the CAS, Prague, Czech Republic.
“The SSX ion source is surely a masterpiece of state of the art engineering. Full understanding of the ion chemistry occurring between the ESI generated ions and VOC molecules inside will be an interesting challenge to tackle.”
Prof. Dr. med. Malcolm Kohler - University Hospital Zurich (USZ), Switzerland.
“We observed significant changes in glyoxylate and dicarboxylate, tricarboxylic acid cycle (TCA), and tryptophan metabolic pathways. Our findings suggest that continuous monitoring of metabolite shifts from exhaled air should focus on monitoring platforms dedicated to volatile and slightly volatile metabolites”
Dr. Prof. Jangjiang Zhu - The Ohio State University, Ohio, USA.
“Our results suggested that the optimized SESI-HRMS method can be suitable forthe analysis of VFAs from gut microbes in a rapid, sensitive, and non-invasive manner.”
Dr. Prof. Renato Zenobi - ETH Zurich, Switzerland.
“When SESI is combined with state-of-the-art mass spectrometers, low limits of detection (pptv) are achievable without any sample pre-concentration. We exploit this feature in our lab in a number of projects which require fast mass spectrometric analysis of vapors at trace concentrations.”
Dr. Prof. Xue Li - Jinan University, Guangzhou, China.
““IAV infection induces a cascade of metabolite alterations, some of which are excreted via the skin and breath to the ambient air. Such IAV-induced odor traits could be captured by SESI-HRMS by simply dragging the air surrounding the infected mice into the analyzer. The process is non-invasive and rapid, as it requires no sample manipulation.”
Dr. Prof. Pablo Sinues - University Children’s Hospital Basel, University of Basel, Switzerland.
“We use SESI-HRMS to sense exhaled molecules at minute concentrations. Since the analysis requires no sample-preparation or manipulation, the diagnostic result can be obtained nearly in real-time. This technology is especially well suited for children due to its non-invasive nature”
Dr. Prof. Lars M. Blank - Institute of Applied Microbiology at RWTH Aachen University, Germany.
“Thank you Fossiliontech for all your support.”
