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Breath molecular Analysis in real time

Identify biologically relevant metabolites

Pharmaco-kinetic profiling made easy

 

Breath analysis offers a unique window
to the human metabolism

 

  • The fact that breath contains larger bio-molecules is well documented.

  • Each time we breath, several thousand molecular species that reflect our metabolism in that particular moment are exhaled.

  • Breath analysis online is non invasive, and can be continuously collected.

 

Breath chemical composition analysis is normally associated with small molecules, like Ethanol, CO2, or NO. Indeed, only a handful breath test are FDA-approved, and they rely on very volatile species:

  • CO2 monitoring (capnography)
  • NO monitoring for asthma therapy
  • Hearts-breath test for heart transplant rejection
  • Urea13C for helicobacter pylori infection diagnosis

In contrast, there are lots of biomarkers with larger molecular masses, which can be detected in other bodily fluids.

 
 
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designed to DETECT RELEVANT
BREATH METABOLITES

More than 2,000 species can be detected in each exhalation

SUPER SESI is built to enable HRMS online analysis of low volatility metabolites in breath.
Thanks to its unique technology, and its ionization mechanism, SUPER SESI meets the requirements
that make analysis of relevant breath metabolites a reality today.

 

MAIN FEATURES

  • Superior sensitivity

  • Highest selectivity

  • Very high throughput

  • Minimalist standardized procedures

  • Improved ionization efficiency to detect minute concentrations

  • Improved background levels and memory effects to detect low volatility species

  • Harness the power of high performance mass spectrometry

  • Online analysis to eliminate handling related cross contamination and improve data quality

 

SUPER SESI KEY COMPONENTS

Our comprehensive solution for molecular analysis of breath
with Mass Spectrometry (SESI-MS)
is composed of

 
  • SUPER SESI. Highly efficient and sensitive ion source compatible with Thermo and Sciex Mass Spectrometers.

  • EXHALION capnography system. It collects the flow exhaled by a person trough a disposable spirometer antibacterial filter, and measures the exhaled flow and CO2 concetration.

  • ARIADNE software. It is a post-processing service specifically designed for SUPER SESI data. It provides an intelligent analysis of the data, and automatically monitors the background levels.

 
 

HOW IT WORKS

  • The patient exhales through a disposable mouth piece, and a constant fraction of the flow is passed to the ionization region.

  • In the ionization region, a nano-Electrospray creates the primary ions, which transfer their charge to the metabolites of interest.

  • Ionized metabolites are efficiently transferred to the mass spectrometer (MS) for analysis.

  • The MS acquires several mass spectra per second. The time evolution of the signals produced by different species can be then tracked separately. This enables the quantification of the different metabolites.

 

 

For more information about the performance of an online breath analysis with SUPER SESI-MS, you can download our Breath Analysis Application Note.


 

 THE RESULT: High quality data

free of artifacts and other confounding variables

 
Plot of the signal intensity vs time of different species found in the volatilome of humans
Breath spectrum in orange, background in purple.

Breath spectrum in orange, background in purple.

  • Obtaining statistically significant data requires measuring large population samples. With SUPER SESI, the acquisition of exhalations is totally non invasive and can be completed in just 10 minutes.

  • With high sensitivity instrumentation, confounding variables can inadvertently sneak in our data set if the acquisition protocol involves too many steps or if samples have to be handled. This could jeopardize an entire breath biomarker
    discovery study.

  • For this reason, SUPER SESI, ionizes the breath samples right as they are produced. Exhalion measures the flows and CO2 concentrations, so that all exhalations are collected and analyzed under the same standardized conditions.


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TIPS TO INCLUDE SUPER SESI IN YOUR BREATH RESEARCH PROTOCOL

A basis breath research plan aimed at the discovery of new breath biomarkers can be categorized as Clinical Study.

We have designed a free guide to help you prepare the corresponding 
Clinical Study Protocol to include SUPER SESI.