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Standardization procedures for real-time breath analysis by secondary electrospray ionization high-resolution mass spectrometry

Kapil Dev Singh, Georgi Tancev, Fabienne Decrue, Jakob Usemann, Rhea Appenzeller, Pedro Barreiro, Gabriel Jaumà, Miriam Macia Santiago, Guillermo Vidal de Miguel, Urs Frey, Pablo ML Sinues

Despite the attractiveness of breath analysis as a non-invasive means to retrieve relevant metabolic information, its introduction into routine clinical practice remains a challenge.

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Xue Li, Dan D. Huang, Rui Du, Zhi J. Zhang, Chak K. Chan, Zheng X. Huang, Zhen Zhou

Exhaled volatile organic compounds (VOCs) have aroused considerable interest, since they can serve as biomarkers for disease diagnosis and environmental exposure in a non-invasive manner. In this work, we present a protocol to characterize the exhaled VOCs in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry (Sec-nanoESI-HRMS).

Translating secondary electrospray ionization–high-resolution mass spectrometry to the clinical environment

Translating secondary electrospray ionization–high-resolution mass spectrometry to the clinical environment

K. D. Singh, G. Vidal-de-Miguel, M. T.s Gaugg, A. J. Ibañez, R. Zenobi, M. Kohler, U. Frey and P. M-L Sinues

While there has been progress in making use of breath tests to guide clinical decision making, the full potential of exhaled breath analysis still remains to be exploited. Here we summarize some of the reasons why this is the case, what we have done so far to overcome some of the existing obstacles, and our vision of how we think breath analysis will play a more prominent role …

Identification of Relatively High Molecular Weight Compounds in Human Breath Using Secondary Nano Electrospray Ionization Ultrahigh Resolution Mass Spectrometry

HUANG Lei, LI Xue, XU Meng, HUANG Zhengxu, ZHOU Zhen

Human breath contains thousands of volatile organic compounds (VOCs), which would be potentially helpful for studying disease diagnosis and environmental exposure. However, owing to limitations in current analytical methods, only the compounds with molecular weights <300 have been widely reported in exhaled human breath.

Direct human breath analysis by secondary nano-electrospray ionization ultrahigh-resolution mass spectrometry: Importance of high mass resolution and mass accuracy

Xue Li, Lei Huang, Hui Zhu and Zhen Zhou.

RATIONALE: Direct mass spectrometry (MS)-based methods make it possible to monitor the molecular compositions of hundreds of volatile organic compounds (VOCs) in exhaled human breath in real time. Mass resolution and mass accuracy play important roles for direct MS analysis, especially for the low-concentration isobaric compounds in non-target research.

Real-Time Chemical Analysis of E-Cigarette Aerosols by Means of Secondary Electrospray Ionization Mass Spectrometry

Real-Time Chemical Analysis of E-Cigarette Aerosols by Means of Secondary Electrospray Ionization Mass Spectrometry

D. Garcia-Gomez, T. Gaisl, C. Barrios-Collado, Guillermo Vidal-de-Miguel, M. Kohler and R. Zenobi

Chemical analysis of aerosols collected from electronic cigarettes (ECs) has shown that these devices produce vapors that contain harmful and potentially harmful compounds. Conventional analytical methods used for the analysis of electronic cigarettes do not reflect the actual composition of the aerosols generated because they usually neglect the changes in the chemical composition that occur during the aerosol generation process and after collection.