breath research

Real-time breath analysis reveals specific metabolic signatures of COPD exacerbations

Real-time breath analysis reveals specific metabolic signatures of COPD exacerbations

Martin Thomas Gaugg, Yvonne Nussbaumer-Ochsner, Lukas Bregy, Malcolm Kohler, Anna Engler, Nina Steblr, Thomas Gaisl, Tobias Bruderer, Nora Nowak, Pablo ML Sinues, Renato Zenobi.

Background
Exacerbations of chronic obstructive pulmonary disease (COPD) are defined by acute worsening of respiratory symptoms leading to a change in therapy.

二次纳流电喷雾电离耦合 超高分辨质谱检测人体呼出气中 相对高分子量化合物

黄 磊, 李 雪, 徐 萌, 黄正旭, 周 振

二次电喷雾电离源耦合超高分辨质谱(SESI⁃UHRMS)有望检出人体呼出气中分子量大于300 的相对 高分子量化合物, 这些化合物的发现将有助于更准确地理解呼出气中挥发性有机化合物(VOCs)的来源、产 生机制以及SESI 源电离机理, 更好地实现SESI⁃UHRMS 的转化应用. 本研究自组装nanoSESI 源(尚无商业产 品)耦合四极杆⁃静电场轨道阱质谱(最高质量分辨率1.2×10<sup>5</sup> ), 考察了该装置对健康人体呼出气中分子量 为300~500 化合物的检出情况. 结果表明, 所搭建的nanoSESI⁃UHRMS 装置检测人体呼出气的重现性好、灵 敏度高, 可检出数十种分子量为300~500 的化合物.

Direct human breath analysis by secondary nano-electrospray ionization ultrahigh-resolution mass spectrometry: Importance of high mass resolution and mass accuracy

Xue Li, Lei Huang, Hui Zhu and Zhen Zhou.

RATIONALE: Direct mass spectrometry (MS)-based methods make it possible to monitor the molecular compositions of hundreds of volatile organic compounds (VOCs) in exhaled human breath in real time. Mass resolution and mass accuracy play important roles for direct MS analysis, especially for the low-concentration isobaric compounds in non-target research.