PK

Real-Time Monitoring of Tricarboxylic Acid Metabolites in Exhaled Breath

Real-Time Monitoring of Tricarboxylic Acid Metabolites in Exhaled Breath

A.Tejero Rioseras, K.D. Singh, N. Nowak, M. T. Gaugg, T. Bruderer, R. Zenobi, P. M-L Sinues

The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates for many other interconnecting pathways and acts as a hub connecting metabolism of carbohydrates, fatty acids, and amino acids.

Comprehensive Real-Time Analysis of the Yeast Volatilome

Comprehensive Real-Time Analysis of the Yeast Volatilome

A. Tejero-Rioseras, D. Garcia-Gomez, B. E. Ebert, L. M. Blank, A. J. Ibanez and P. M-L Sinues

While yeast is one of the most studied organisms, its intricate biology remains to be fully mapped and understood. This is especially the case when it comes to capture rapid, in vivo fluctuations of metabolite levels. According to the authors, the results suggest that a large number of metabolites produced by yeast from glucose neither are reported in the literature nor are their biochemical origins deciphered.

Metabolic effects of inhaled salbutamol determined by exhaled breath analysis

Metabolic effects of inhaled salbutamol determined by exhaled breath analysis

M. T. Gaugg, A. Engler, Y. Nussbaumer-Ochsner, L. Bregy, A. S Stöberl, T. Gaisl, T. Bruderer, R. Zenobi, M. Kohler and P. M-L Sinues

The authors explore whether real-time breath research & analysis by SESI-HRMS is suitable to monitor changes at the metabolic level due to inhaling bronchodilator medication. The experimental results strengthen the notion that certain  biochemical processes can be monitored.

Gauging circadian variation in ketamine metabolism by real-time breath analysis

Gauging circadian variation in ketamine metabolism by real-time breath analysis

P. M-L Sinues, M. Kohler, S. A. Brown, R. Zenobi and R. Dallmann

The time-of-day of drug application is an important factor in maximizing efficacy and minimizing toxicity. Real-time in vivo mass spectrometric breath analysis and research of mice was deployed to investigate time-of-day variation in ketamine metabolism. Different production rates of ketamine metabolites were found in opposite circadian phases.

Real-Time Quantification of Amino Acids in the Exhalome by SESI-MS: A Proof-of-Principle Study

Real-Time Quantification of Amino Acids in the Exhalome by SESI-MS: A Proof-of-Principle Study

D. García-Gómez, T. Gaisl, L. Bregy, A. Cremonesi, P. M-L Sinues, M. Kohler, and R. Zenobi

Amino acids are frequently determined in clinical chemistry. However, current analysis methods are time-consuming, invasive, and require sample preparation. The authors hypothesized that plasma concentrations of amino acids can be estimated by measuring their concentrations in exhaled breath.