The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates for many other interconnecting pathways and acts as a hub connecting metabolism of carbohydrates, fatty acids, and amino acids.
We aimed at defining profiles of volatile organic compounds in exhaled breath from patients with cystic fibrosis (CF) using a novel real-time mass spectrometry technique. In this prospective matched case-control study, 30 patients with CF, and 30 healthy control 4 subjects were matched one-to-one according to age, gender, and smoking state.
While there has been progress in making use of breath tests to guide clinical decision making, the full potential of exhaled breath analysis still remains to be exploited. Here we summarize some of the reasons why this is the case, what we have done so far to overcome some of the existing obstacles, and our vision of how we think breath analysis will play a more prominent role …
The authors explore whether real-time breath research & analysis by SESI-HRMS is suitable to monitor changes at the metabolic level due to inhaling bronchodilator medication. The experimental results strengthen the notion that certain biochemical processes can be monitored.
Coupling a SESI source and a HRMS (Orbitrap), the authors were able to identify exogenous compounds associated to smoking, as well as endogenous metabolites suggesting increased oxidative stress in smokers. According to the authors, most of these compounds correlated significantly with smoking frequency and allowed accurate discrimination of smokers and non-smokers.
SESI–MS produces high-dimensional data of quality comparable to gene expression analysis output. This prompted the authors to use methods originally developed for gene expression data in analyzing SESI–MS breathprints.
H. D. Bean, J. Zhu, J. C. Sengle and J. E. Hill
Invasive methicillin-resistant Staphylococcus aureus (MRSA) infections are a serious health threat, causing an estimated 11000 deaths per year in the United States. MRSA pneumonias account for 16% of invasive infections, and can be difficult to detect as the current state-ofthe-art diagnostics require that bacterial DNA is recovered from the infection site …
J. Zhu, J. Jimenez-Díaz, H. D. Bean, N. A. Daphtary, M. I. Aliyeva, L. K. A. Lundblad and J. E. Hill
Before breath-based diagnostics for lung infections can be implemented in the clinic, it is necessary to understand how the breath volatiles change during the course of infection, and ideally, to identify a core set of breath markers that can be used to diagnose the pathogen at any point during the infection …
P. M-L Sinues, L. Zingaro, A. Finiguerra and S. Cristoni
A series of fatty acids among other compounds have recently been detected in breath in real time by secondary electrospray ionization mass spectrometry (SESI-MS). Our main aim in this work was to quantify their abundance in breath calibrating the system with standard vapors and extend the study to a control group for several days, both under fasting conditions and after sucrose intake.