Journal Breath Research

Real-Time Monitoring of Tricarboxylic Acid Metabolites in Exhaled Breath

Real-Time Monitoring of Tricarboxylic Acid Metabolites in Exhaled Breath

A.Tejero Rioseras, K.D. Singh, N. Nowak, M. T. Gaugg, T. Bruderer, R. Zenobi, P. M-L Sinues

The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates for many other interconnecting pathways and acts as a hub connecting metabolism of carbohydrates, fatty acids, and amino acids.

Real-time exhaled breath analysis in patients with cystic fibrosis and controls

Real-time exhaled breath analysis in patients with cystic fibrosis and controls

T. Gaisl, L. Bregy, N. Stebler, M. T. Gaugg, T. Bruderer, D. García-Gómez, A, Moeller,  F. Singer,  E. I.  Schwarz, C. Benden, P. M-L Sinues, R. Zenobi, M. Kohler

We aimed at defining profiles of volatile organic compounds in exhaled breath from patients with cystic fibrosis (CF) using a novel real-time mass spectrometry technique. In this prospective matched case-control study, 30 patients with CF, and 30 healthy control 4 subjects were matched one-to-one according to age, gender, and smoking state.

Translating secondary electrospray ionization–high-resolution mass spectrometry to the clinical environment

Translating secondary electrospray ionization–high-resolution mass spectrometry to the clinical environment

K. D. Singh, G. Vidal-de-Miguel, M. T.s Gaugg, A. J. Ibañez, R. Zenobi, M. Kohler, U. Frey and P. M-L Sinues

While there has been progress in making use of breath tests to guide clinical decision making, the full potential of exhaled breath analysis still remains to be exploited. Here we summarize some of the reasons why this is the case, what we have done so far to overcome some of the existing obstacles, and our vision of how we think breath analysis will play a more prominent role …

Metabolic effects of inhaled salbutamol determined by exhaled breath analysis

Metabolic effects of inhaled salbutamol determined by exhaled breath analysis

M. T. Gaugg, A. Engler, Y. Nussbaumer-Ochsner, L. Bregy, A. S Stöberl, T. Gaisl, T. Bruderer, R. Zenobi, M. Kohler and P. M-L Sinues

The authors explore whether real-time breath research & analysis by SESI-HRMS is suitable to monitor changes at the metabolic level due to inhaling bronchodilator medication. The experimental results strengthen the notion that certain  biochemical processes can be monitored.

Expanding metabolite coverage of real-time breath analysis by coupling a universal SESI source and a HRMS. A pilot study on tobacco smokers

Expanding metabolite coverage of real-time breath analysis by coupling a universal SESI source and a HRMS. A pilot study on tobacco smokers

M. T. Gaugg, D. Garcia Gomez, C. Barrios-Collado, G. Vidal-de-Miguel, M. Kohler, R. Zenobi and P. M-L Sinues

Coupling a SESI source and a HRMS (Orbitrap), the authors were able to identify exogenous compounds associated to smoking, as well as endogenous metabolites suggesting increased oxidative stress in smokers. According to the authors, most of these compounds correlated significantly with smoking frequency and allowed accurate discrimination of smokers and non-smokers.

SESI-MS and a novel statistical bioinformatic approach identifies a cancer-related profile in exhaled breath of breast cancer patients: a pilot study

SESI-MS and a novel statistical bioinformatic approach identifies a cancer-related profile in exhaled breath of breast cancer patients: a pilot study

P. M-L Sinues, Elena Landoni, Rosalba Miceli, Vincenza F. Dibari, Matteo Dugo, Roberto Agresti, Elda Tagliabue, Simone Cristoni, Rosaria Orlandi.

SESI–MS produces high-dimensional data of quality comparable to gene expression analysis output. This prompted the authors to use methods originally developed for gene expression data in analyzing SESI–MS breathprints.

Identifying methicillin-resistant Staphylococcus aureus (MRSA) lung infections in mice via breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS)

Identifying methicillin-resistant Staphylococcus aureus (MRSA) lung infections in mice via breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS)

H. D. Bean, J. Zhu, J. C. Sengle and J. E. Hill

Invasive methicillin-resistant Staphylococcus aureus (MRSA) infections are a serious health threat, causing an estimated 11000 deaths per year in the United States. MRSA pneumonias account for 16% of invasive infections, and can be difficult to detect as the current state-ofthe-art diagnostics require that bacterial DNA is recovered from the infection site …

Robust detection of P. aeruginosa and S. aureus acute lung infections by secondary electrospray ionization-mass spectrometry (SESI-MS) breathprinting: from initial infection to clearance

Robust detection of P. aeruginosa and S. aureus acute lung infections by secondary electrospray ionization-mass spectrometry (SESI-MS) breathprinting: from initial infection to clearance

J. Zhu, J. Jimenez-Díaz, H. D. Bean, N. A. Daphtary, M. I. Aliyeva, L. K. A. Lundblad and J. E. Hill

Before breath-based diagnostics for lung infections can be implemented in the clinic, it is necessary to understand how the breath volatiles change during the course of infection, and ideally, to identify a core set of breath markers that can be used to diagnose the pathogen at any point during the infection …

Secondary electrospray ionization-mass spectrometry: breath study on a control group

Secondary electrospray ionization-mass spectrometry: breath study on a control group

P. M-L Sinues, L. Zingaro, A. Finiguerra and S. Cristoni

A series of fatty acids among other compounds have recently been detected in breath in real time by secondary electrospray ionization mass spectrometry (SESI-MS). Our main aim in this work was to quantify their abundance in breath calibrating the system with standard vapors and extend the study to a control group for several days, both under fasting conditions and after sucrose intake.