Real-time metabolomics

Every living organism breathes in one way or another. We do it between 17,000 and 30,000 times every day.
Each exhalation carries several thousand molecular species that reflect our metabolism in that particular moment.
Breath research via SUPER SESI is non invasive, and can be continuously monitored, offering an excellent window to the metabolism.

 

selected scientific literature:
- Real time metabolomics -


Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles

 Time traces for β caryophyllene and indole illustrate a typical diurnal and nocturnal pattern.

Time traces for β caryophyllene and indole illustrate a typical diurnal and nocturnal pattern.

C. Barrios-Collado, D. Garcia-Gomez, R. Zenobi, G. Vidal-de-Miguel, Alfredo J. Ibanez, and P. M-L Sinues

The authors document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day−night cycles. To further illustrate the capabilities of SESI-MS for volatile organic compounds (VOCs) analysis, they subjected the plant to mechanical damage and monitored its response.


Comprehensive Real-Time Analysis of the Yeast Volatilome

 Kinetic profiles of the food-relevant metabolite for different baker's yeast mutations illustrates the potential of SESI-HRMS to monitor industrial processes.

Kinetic profiles of the food-relevant metabolite for different baker's yeast mutations illustrates the potential of SESI-HRMS to monitor industrial processes.

A. Tejero Rioseras, D. Garcia Gomez, B. E. Ebert, L. M. Blank, A. J. Ibanez and P. M-L Sinues

While yeast is one of the most studied organisms, its intricate biology remains to be fully mapped and understood. This is especially the case when it comes to capture rapid, in vivo fluctuations of metabolite levels. According to the authors, the results suggest that a large number of metabolites produced by yeast from glucose neither are reported in the literature nor are their biochemical origins deciphered.


Gauging circadian variation in ketamine metabolism by real-time breath analysis

 Metabolism of norketamine (NK) depending on internal time, two measures in the evening and another two in the opposite circadian phase.

Metabolism of norketamine (NK) depending on internal time, two measures in the evening and another two in the opposite circadian phase.

P. M-L Sinues, M. Kohler, S. A. Brown, R. Zenobi and R. Dallmann

The time-of-day of drug application is an important factor in maximizing efficacy and minimizing toxicity. Real-time in vivo mass spectrometric breath analysis and research of mice was deployed to investigate time-of-day variation in ketamine metabolism. Different production rates of ketamine metabolites were found in opposite circadian phases.


Rapid fingerprinting of grape volatile composition using SESI orbitrap MS: A preliminary study of grape ripening

 Principal component analysis score plot for first and second components for three grape cultivars

Principal component analysis score plot for first and second components for three grape cultivars

R. R. Farrell, J. Fahrentrapp, D. Garcia Gomez, P. M-L Sinues, and R. Zenobi

Even though sugar and acidity measurements are the most common indices of grape maturity, it is well recognized that they provide only basic information related to wine quality. In this preliminary study te authors use SESI-MS to analyze VOCs directly from intact grapes without sample concentration.


SUPER SESI USE CASES